Though the listed allowances

Though the listed allowances idealise a situation, but they approximately outline conditions of radio immunologic researches. By means of corresponding methods it is possible to define such concentration of hormones which usually do not give in to definition by the majority of biological methods in vivo. As sensitivity or the least definable dose understand that concentration of a hormone which causes significant change of percent of linkage of a labelled hormone with antibodies at comparison about "empty" breakdown. Sensitivity order ultram zoloft of definition depends on affinity of a hormone to specific antibodies. Most widely applied method fibers is, possibly, modified method offered Greenwood, Hunter and Glover [27]. In it the oxidising agent Chloraminum transforming Na125I in free iodine which then joins mainly in Thyrosinum rests is used. At use of this method can and Histidinum rests. For conservation of the maximum immunoreactivity] it is necessary, that on a hormone molecule one atom 131I or 125I as the big degree usually reduces immunologic and biological activity of a hormone had only, changing its chemical properties [27]. Now apply also other ways .
The place second for popularity occupies, apparently, an enzymatic method with lactoperoxidase use [28, 29]. In the presence of the peroxide of hydrogen added immediately in a reactionary admixture, the lactoperoxidase transforms Na125I into free iodine which selectively joins in Thyrosinum rests. As hydrogen peroxide is a strong oxidizer, action of its high concentration can change its chemical properties to a hormone; therefore hydrogen peroxide usually add in two or three small portions which are quickly used in reaction. Another, but less widespread method , the method developed Bolton and Hunter [30] is newer. In it possibility of harmful effects of Chloraminum , bisulphite of sodium and peroxide of hydrogen, applied in two other methods , on a hormone is reduced to a minimum. By means of Chloraminum pyridium 200mg the intermediate bond-ester, formed N-oksisuktsinimidom and 3 (r-oksifenil)-propionic acid which then is condensed with amino groups of a polypeptide hormone. Such approach allows to avoid influence on a hormone of strong oxidizers or reducers. This technique is a little bulky and yet has not received a wide circulation. Now use not Na131I, a Na125I as it possesses considerably the big half-life period: 59 days instead of 6. However at work with 125I special carefulness, as its specific g-activity much more low, than at 131I is necessary. Steroid hormones and vitamin D mark , that allows to receive higher specific activity, than by means of a label 14C. The polypeptides which are not incorporating the rests of Thyrosinum or Histidinum, it is possible to modify, entering or attaching Thyrosinum to their structure without visible change of conformstion of native bond. Choose such replacements which with the least probability will change conformstion of a molecule. ANTISERUM RECEPTION Antibodies belong to a class of immunoglobulins and are mainly g-globulins (IgG). Molecule IgG consists of 4 polypeptide chains: two serious and two easy, symmetrized and covalently bound with each other disulfide ponticuluses. The schematic image of this molecule is resulted on 5-2. Antigen interaction, or , with an antibody can assimilate contact the latch and a key. The form an antibody site that of an antigen site. Communication between an antigen and an antibody is caused by a combination electrostatic, hydrogen and van-der-vaalsovyh interactions [31, 32]. The form of a binding site and by that affinity and specificity of communication of molecule IgG with the necessary antigen is defined by amino-acid sequence a site of this molecule [33]. For reception of system of definition of a hormone a high cleaning hormone enter a laboratory animal, inducing at them synthesis of the immunoglobulins possessing enough high specificity and sensitivity that they could be used for radio immunologic definitions. As a rule, less than 1000 dalton is not enough bond with the sizes of molecules and for giving of properties by it active they need to be bridged to a molecule-carrier. As an immunogen is called the substance, capable to induce formation of antibodies. To provide possibility of an induction of an antibodyformation, small molecules, or , it is possible to bridge to such substances, as an albumin, or a hemocyanin. reaction usually above in case of introduction of those fibers-carriers which and are . In this connection such substances as an albumin, and a hemocyanin the polylysine or other synthetic polyamino acids are more suitable fibers-carriers, than, at an induction of synthesis of specific antibodies to . Steroids, thyroid hormones ( and a thyroxine) and various forms of vitamin D, as a rule, demand a conjugation with fibers-carriers. It is necessary to watch that in the course of a conjugation of steroids and others that part of the molecule which change least would affect stereospecificity of this molecule took part carefully. For example, antibodies to 17 ? to-Oestradiolum is better are formed at its covalent joining 6-ketoanaloga to an albumin, i.e. At conservation 3 and 17-hydroxylic groups, no less than a ring And, a steroid.