The last is especially

The last is especially important, as these structures give to the given estrogen specificity. For many years various techniques of immunization are developed, but all of them, in essence, are updatings of original technique [34]. The emulsion decadron containing a hormone, or an immunogen, corresponding oil and the tubercular rods killed by warming, enter intra-or subcutaneously. With reception of high cleaning hormones and other derivatives it was possible to develop an immunization technique small quantities of an immunogen [35]. Reliable experimental proofs of education of specific antibodies possessing high affinity to small doses of an immunogen [36] are had. Small doses of an immunogen with the greatest probability cause production of specific immunoglobulins those cells which form antibodies with the highest affinity to an immunogen. In this connection at application of the big doses of an immunogen the probability of development of the antibodies possessing low affinity raises. At introduction of very big doses of an immunogen at animals the tolerance phenomenon when antibodies are not formed or formed at all in very low caption [37, 38] can be observed. After initial immunization usually should pass a minimum 6 that there were appreciable antiserum capacities. Affinity of antibodies continues to be enlarged and reaches a maximum usually between 8th and 12th week after initial immunization [35, 39]. It is empirically established, that repeated immunization of an animal provides the best reaction in that if repeated injections make in depression circulating antibodies.
At repeated introduction of 1/2 or 1/4 doses of the hormone, used at primary immunization, substantial increase circulating antibodies is observed in some days. Repeated immunization of an animal should be made in the same anatomic area into which an immunogen entered first time. It is more than that, for reaction reproduction on memory it is not so necessary to enter the tubercular bacilli killed by warming which can enlarge a case rate and a mortality of animals. For reception of specific antibodies it is necessary to choose those kinds of animals which are easily accessible, not too expensive and at which the structure avandamet of an own endogenous hormone considerably differs from applied to immunization. For example, though at animal different kinds possess identical biological activity, but they essentially differ on amino-acid structure. These differences in primary structure, as well as some other insufficiently characterised factors define an adjuvanticity of a hormone for other kinds of animals. In some cases amino-acid sequences can differ rather weakly and consequently it is necessary to select a kind of an animal to which enter a hormone with special carefulness. For example, the structure of insulin at the person, a rabbit and a sheep is almost identical, but insulins of the person and a porpoise differ more strongly. For reception of specific antibodies to insulin of the person is better to use porpoises [40]. SENSITIVITY OF SYSTEM OF DEFINITION After reception of antibodies from an animal and definition of their concentration spend an estimation of sensitivity and specificity of an antiserum. Concentration of antibodies in Serum in the beginning define on linkage of a labelled hormone which applied to immunization, and use different delutions of an antiserum. This way is called as titration and under understand concentration of molecules of antibodies in Serum. After a finding of that concentration of antibodies which in equilibrium conditions binds 30-40 of a labelled hormone, define sensitivity and specificity of antibodies. Reception and the characteristic of specific systems for definition and insulin it is very in detail stated in other publications and can serve as a reference point at reception of other specific radio immunologic systems of definition of hormones. In real conditions the antibodies received for use in clinical radio immunologic researches, contain population of the immunoglobulins possessing various specificity and affinity. It is necessary to choose an antiserum containing population of antibodies with high enough affinity and specificity to that hormone which should be defined. In practice to choose such population of antibodies with high affinity high delution of an antiserum that the role of other populations of the antibodies possessing the lowest affinity and, probably, nonspecific, became concerning insignificant is possible by enough. At a choice of such delution of an antiserum which differs from used in the beginning for the system characteristic, it is necessary to define anew its sensitivity and specificity as in other delution concentration of other populations of antibodies can appear concerning high, and the divorced antiserum will possess smaller sensitivity and specificity.