Reaction "Deenergizing"

Reaction "Deenergizing" is blocked by not physiological agents, such as Gpp (NH) p and choleraic toxin, and also can serve as way of regulation of activity of adenylatecyclase other intracellular signals. The further indicatings on value a site have been received at reconstruction attempts activity of adenylatecyclase by cell fusion or order ultram online addition of soluble cellular extracts to to membranes. In experiences on cell fusion Heterokarionums, from characteristic for blood cells (deprived b-receptors) and erythrocytes of a turkey (with adenylatecyclase), found out the activity which was absent in donor cells [35]. Like it, receptors containing in extracts rich with lipids of ovaries, have been transferred to cells of a mesh zone of a cortex of adrenals in which they mediated action of Gonadotropinums on production and corticosteroids [6]. In experiments on reconstruction a detergent adenylatecyclase joined in structure of membranes of mutant cells containing receptors, scarce on adenylatecyclase, with restoration of activity of enzyme stimulated with catecholamins [36]. Like the mentioned results of researches with cell fusion these data underline spatial independence of hormonal receptors and adenylatecyclase and testify to ability of receptors to movement in a direction and to interaction with adenylatecyclase unit in a cellular membrane.
Such data testify that between hormonal receptors and components systems there is the general interfacing mechanism shown at interaction of receptors and adenylatecyclase of a cell-owner. It could specify in existence of area of conformation similarity at all receptors hormones at conservation of uniqueness of their areas responsible for specific linkage of hormones [36]. To finding-out of a question on, whether really receptors hormones possess the general domain defining possibility of interaction with by components of membranous enzymes, more detailed structural analysis of these receptors should vpxl herbal promote. Results of experiences on the reconstruction spent on mutant cells, possessing b-adrenergic receptors and adenylatecyclase, which though was present, but has been separated"(i.e. Did not react to catecholamins), have shown restoration of activity stimulated with catecholamins at addition of extracts of cells of wild type. It testifies that the interfacing factor (it is possible, normal unit), joining in a membrane of a mutant cell, replaces presumably defective at such mutants a site [36]. Besides participation in adenylatecyclase activation, guanylic nucleotides often influence and on linkages and affinity interactions. The analysis membranous systems and separate effects of guanylic nucleotides on linkage of a hormone and cyclase activation specifies in structural and functional isolation of the sites influencing conformstion of a receptor and enzymatic activity [32]. "receptor" and "catalytic" unit the squirrel is reliably proved by results of these and earlier researches, but the question on the mechanism of interaction of components of reaction remains unresolved. A prominent aspect of influence on affinity of receptors to is ability of a nucleotide to reduce "durability" of linkage of a hormone almost from irreversible association to a condition (in some cases) with ready smaller affinity. It was observed concerning glucagon receptors, and also concerning linkage of ligandov-agonists with b-adrenergic receptors. Unlike it, guanylic nucleotides did not change linkage b-antagonists that specifies in a role of interface to adenylatecyclase in ability of nucleotides to raise dissociation of the agonist bound to a receptor [20]. Though the data received at research of system, testify in favour of existence of the isolated scenes of action of nucleotides on regulation of receptor linkage and activity of adenylatecyclase, however a role of these places in hormonal activation and interface still remains not found out. The condition of low affinity of the receptor, caused , is surveyed as a favorable previous condition of process of transmembrane interface, and the separated form of a receptor possessing high affinity, could reflect an inactive condition of enzyme. The last could concern to adenylatecyclases the raised concentration of a hormone [20]. Role in effects : protein kinases After opening and the analysis of its role in effects of a glucagon and adrenaline on a glycolysis it has been found out, that the cyclic nucleotide takes part in regulation of various metabolic functions. As the certificate that is served by global presence at and and its participation in the reactions bound not only with action of hormones [4]. It has been established, that ability to activate in hepatic cells is the general property cells in which all effects are realised through albuminous substrates. Similarly in cells there is an enzyme named a tsamf-dependent protein kinase which mediates effects on a cellular metabolism [37]. At effects are realised through other mechanism - interaction of tsamf-binding fibers with sites . In animal tissues a tsamf-dependent protein kinase many albuminous substrates, transferring Natrii phosphas with on specific (and sometimes ) the rests. In the given place a number of known albuminous substrates, including enzymes, such, as , and a lipase, and not fermental cellular fibers, such, as histones, nuclear , ribosomal fibers, fibers of microtubules and membranes [38].